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A gDNA Microarray for Genotyping Salvia Species.

Mol Biotechnol. 2012 Dec 27;
Olarte A, Mantri N, Nugent G, Wohlmuth H, Li CG, Xue C, Pang E

Salvia is an important genus from the Lamiaceae with approximately 1,000 species. This genus is distributed globally and cultivated for ornamental, culinary, and medicinal uses. We report the construction of the first fingerprinting array for Salvia species enriched with polymorphic and divergent DNA sequences and demonstrate the potential of this array for fingerprinting several economically important members of this genus. In order to generate the Salvia subtracted diversity array (SDA) a suppression subtractive hybridization (SSH) was performed between a pool of Salvia species and a pool of angiosperms and non-angiosperms to selectively isolate Salvia-specific sequences. A total of 285-subtracted genomic DNA (gDNA) fragments were amplified and arrayed. DNA fingerprints were obtained for fifteen Salvia genotypes including three that were not part of the original subtraction pool. Hierarchical cluster analysis indicated that the Salvia-specific SDA was capable of differentiating S. officinalis and S. miltiorrhiza from their closely related species and was also able to reveal genetic relationships consistent with geographical origins. In addition, this approach was capable of isolating highly polymorphic sequences from chloroplast and nuclear DNA without preliminary sequence information. Therefore, SDA is a powerful technique for fingerprinting non-model plants and for identifying new polymorphic loci that may be developed as potential molecular markers.

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A new culinary school takes shape in San Francisco
With Daniel Patterson, Craig Stoll, Bill Corbett and Jodi Liano at the helm, the new San Francisco Cooking School makes a formidable entry onto the Bay Area's culinary scene.
Mon, 07 Jan 2013 15:03:46 -0800
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Herbs, health and hot tips
src=” http://gardenezi.files.wordpress.com/2013/01/herbs-003.jpg?w=294 alt=”A fine pot of mint” width=”294″ height=”300″ class=”size-medium wp-image-628″ /> A fine pot of mint[/caption] Freshly-potted herbs: basil, sage, parsley and thyme growing in a sunny position on a patio A well-establis
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#art #jobs Culinary Arts Faculty - ECPI University - Norfolk, VA http://t.co/SWLSwu7Q
#art #jobs Culinary Arts Faculty - ECPI University - Norfolk, VA http://t.co/SWLSwu7Q
AmericaHires@twitter.com (America Hires)
Tue, 08 Jan 2013 01:55:28 +0000
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